


| 产品编号 | 产品名称 | 包装 |
| C3601-1 | 线粒体分离试剂 | 125ml |
| C3601-2 | 台盼蓝染色液 | 10ml |
| C3601-3 | 线粒体储存液 | 15ml |
| C3601-4 | 线粒体裂解液 | 15ml |
| C3601-5 | PMSF(晶体) | For 1.5ml 100mM |
| C3601-6 | PMSF(溶剂) | 1.5ml |
| — | 说明书 | 1份 |
保存条件:
-20℃保存,一年有效。其中台盼蓝染色液也可以4℃保存,PMSF(晶体)和PMSF(溶剂)在配制成100mM PMSF溶液前可以室温保存。
注意事项:
试剂盒中的试剂对于不同的实验目的不必全部使用。
如果不是用于制备线粒体蛋白样品,线粒体分离试剂和线粒体裂解液中不必加入PMSF。可以向碧云天订购。PMSF一定要在线粒体分离试剂或线粒体裂解液加入到样品中前2-3分钟内加入,以免PMSF在水溶液中很快失效。
如果用于制备线粒体蛋白样品,线粒体分离试剂和线粒体裂解液中需添加PMSF。PMSF一定要在线粒体分离试剂或线粒体裂解液加入到样品中前2-3分钟内加入,以免PMSF在水溶液中很快失效。
分离线粒体的所有步骤均需在冰上或4℃进行,所用溶液需冰浴或4℃预冷。
通常在分离线粒体时前后两次离心速度选取600g和11,000g,如果希望纯度更高,但对线粒体的得率要求不高,前后两次离心速度可以采用1000g和3500g。
使用本试剂盒中的线粒体储存液稀释或储存的线粒体样品应及时使用,以免线粒体膜电位受影响。如果不能及时使用,建议在-80℃保存。冻存后的线粒体样品不推荐用于膜电位的检测,但可以用线粒体蛋白或核酸的相关检测。
如果出现本试剂盒中线粒体储存液不够用的情况,可以单独订购线粒体储存液(C3609)。
台盼蓝与PMSF对人体有毒,操作时请特别小心,并注意有效防护以避免直接接触人体或吸入体内。
Bradford法蛋白浓度测定试剂盒(P0006)、Bradford蛋白浓度测定试剂盒(去垢剂兼容型)(P0006C)和BCA蛋白浓度测定试剂盒(P0009/P0010/P0010S/P0011/P0012/P0012S)可以向碧云天订购。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。
使用说明:
1. 准备溶液:室温融解试剂盒中的各种溶液,融解后立即置于冰上并混匀。第一次使用时,把1.5ml PMSF(溶剂)加入到PMSF(晶体)中,溶解并混匀,即得到1.5ml 100mM PMSF。配制好的100mM PMSF溶液-20℃保存。如果最终目的是制备线粒体蛋白样品,根据样品数量,取适量线粒体分离试剂备用,在线粒体分离试剂加入到细胞样品中前数分钟内加入PMSF,使PMSF的最终浓度为1mM。同时,根据样品数量取适量线粒体裂解液备用,在线粒体裂解液加入到线粒体样品中前数分钟内加入PMSF,使PMSF的最终浓度为1mM。
2. 收集细胞:
对于贴壁细胞:用PBS洗一遍,用胰酶细胞消化液(Trypsin-EDTA Solution)消化细胞,100-200g,室温离心5-10分钟收集细胞。胰酶细胞消化液(C0201)可以向碧云天订购。
对于悬浮细胞:直接离心收集细胞。
3. 洗涤细胞:用冰浴预冷的PBS轻轻重悬细胞沉淀,取少量细胞用于计数,剩余细胞600g,4℃离心5分钟沉淀细胞。弃上清。
4. 预处理:加入1-2.5ml线粒体分离试剂或临用前添加了PMSF的线粒体分离试剂至2000-5000万细胞中,轻轻悬浮细胞,冰浴放置10-15分钟。
5. 匀浆:把细胞悬液转移到一适当大小的玻璃匀浆器中,匀浆10-30下左右。
6. 匀浆效果的鉴定:不同细胞不同匀浆器所需的匀浆次数有所不同,需自行优化。通常可以在匀浆10次后取约2微升细胞匀浆,加入30-50微升台盼蓝染色液,混匀后显微镜下观察台盼蓝染色阳性(蓝色)细胞的比例。如果阳性细胞比例不足50%,增加5次匀浆。随后再同前取样进行台盼蓝染色鉴定。当阳性比例超过50%时即可停止匀浆进入下一步。请勿过度匀浆,过度匀浆会导致线粒体的机械损伤。同时记录对于该细胞的匀浆次数,通常在后续实验时不必再摸索匀浆次数。
7. 把细胞匀浆在600g,4℃离心10分钟。
注:如需获得纯度更高的线粒体,可以将此步骤的离心速度改为1000g,其它离心条件不变;获得更高纯度线粒体的缺点是相同数量细胞的线粒体抽提得率会下降。
8. 小心把上清转移到另一离心管中,在11,000g,4℃离心10分钟。
注:如需获得纯度更高的线粒体,可以将此步骤的离心速度改为3500g,其它离心条件不变;获得更高纯度线粒体的缺点是相同数量细胞的线粒体抽提得率会下降。
9. 小心去除上清。沉淀即为分离得到的细胞线粒体。
注:如果希望获得去除线粒体的细胞浆蛋白,在本步骤需收集上清,并且在收集上清时注意勿触及沉淀。随后把收集的上清12,000g,4℃离心10分钟。取上清即为去除线粒体的细胞浆蛋白。该细胞浆蛋白可以使用BCA法或Bradford法测定蛋白浓度。
10. 线粒体的使用:
a. 如果用于完整线粒体的功能或酶活性研究,初始2000-5000万细胞分离得到的线粒体样品中可以加入150-200微升线粒体储存液,重悬线粒体。
b. 如果用于线粒体的蛋白分析,初始2000-5000万细胞分离得到的线粒体样品中可以加入150-200微升临用前添加了PMSF的线粒体裂解液裂解线粒体。裂解后的线粒体可以用于PAGE、Western、IP以及线粒体中的一些酶活性的测定等。裂解后的蛋白样品可以使用BCA法或去垢剂兼容型Bradford法(P0006C)测定蛋白浓度。
c. 如果用于双向电泳,请使用适当的用于双向电泳的裂解液处理线粒体。
相关产品:
| 产品编号 | 产品名称 | 包装 |
| C2006 | 线粒体膜电位检测试剂盒(JC-1) | >100次 |
| C3601 | 细胞线粒体分离试剂盒 | 50-100次 |
| C3606 | 组织线粒体分离试剂盒 | 50-100次 |
| C3609 | 线粒体储存液 | 50ml |
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CSE/H2S system protects mesenchymal stem cells from hypoxia and serum deprivation induced apoptosis via mitochondrial injury, endoplasmic reticulum stress and PI3K/Akt activation pathways.
Mol Med Rep. 2015 Aug;12(2):2128-34.
67.Jiang L, Li L, He X, Yi Q, He B, Cao J, Pan W, Gu Z.
Overcoming drug-resistant lung cancer by paclitaxel loaded dual-functional liposomes with mitochondria targeting and pH-response.
Biomaterials. 2015 Jun;52:126-39.
68.Wang J, Gao Q, Wang D, Wang Z, Hu C.
Metformin inhibits growth of lung adenocarcinoma cells by inducing apoptosis via the mitochondria mediated pathway.
Oncol Lett.2015 Sep;10(3):1343-1349.
69.Cai XY, Xia Y, Yang SH, Liu XZ, Shao ZW, Liu YL, Yang W, Xiong LM.
Ropivacaine-and bupivacaine-induced death of rabbit annulus fibrosus cells in vitro: involvement of the mitochondrial apoptotic pathway.
Osteoarthritis Cartilage.2015 Oct;23(10):1763-75.
70.Lu M, Sun L, Zhou J, Zhao Y, Deng X.
Dihydroartemisinin-Induced Apoptosis is Associated with Inhibition of Sarco/Endoplasmic Reticulum Calcium ATPase Activity in Colorectal Cancer.
Cell Biochem Biophys. 2015 Sep;73(1):137-45.
71.Wen X, Zhou J, Zhang D, Li J, Wang Q, Feng N, Zhu H, Song Y, Li H, Bai C.
Denatonium inhibits growth and induces apoptosis of airway epithelial cells through mitochondrial signaling pathways.
Respir Res. 2015 Feb 5;16:13.
72.Huang G, Mao J, Ji Z, Ailati A.
Stachyose-induced apoptosis of Caco-2 cells via the caspase-dependent mitochondrial pathway.
Food Funct.2015 Mar;6(3):765-71.
73.Sun W, Zhang X, Ding X, Li H, Geng M, Xie Z, Wu H, Huang M.
Lactate Dehydrogenase B Is Associated with the Response to Neoadjuvant Chemotherapy in Oral Squamous Cell Carcinoma.
PLoS One.2015 May 14;10(5):e0125976.
74.Cao L, Chen J, Li M, Qin YY, Sun M, Sheng R, Han F, Wang G, Qin ZH.
Endogenous level of TIGAR in brain is associated with vulnerability of neurons to ischemic injury.
Neurosci Bull.2015 Oct;31(5):527-40.
75.Zhang BF, Xing L, Cui PF, Wang FZ, Xie RL, Zhang JL, Zhang M, He YJ, Lyu JY, Qiao JB, Chen BA, Jiang HL.
Mitochondria apoptosis pathway synergistically activated by hierarchical targeted nanoparticles co-delivering siRNA and lonidamine.
Biomaterials.2015 Aug;61:178-89.
76.Li X, Liu X, Xu Y, He Y, Liu J, Xie M.
Expression profile of apoptotic and proliferative proteins in hypoxic HUVEC treated with statins.
Int J Oncol. 2015 Feb;46(2):677-84.
77.Gong Y, Ni ZH, Zhang X, Chen XH, Zou ZM.
Valproic Acid Enhances the Anti-tumor Effect of (-)-gossypol to Burkitt Lymphoma Namalwa Cells.
Biomed Environ Sci. 2015 Oct;28(10):773-7.
78.Zheng G, Lyu J, Liu S, Huang J, Liu C, Xiang D, Xie M, Zeng Q.
Silencing of uncoupling protein 2 by small interfering RNA aggravates mitochondrial dysfunction in cardiomyocytes under septic conditions.
Int J Mol Med.2015 Jun;35(6):1525-36.
79.Deng S, Tang S, Zhang S, Zhang C, Wang C, Zhou Y, Dai C, Xiao X.
Furazolidone induces apoptosis through activating reactive oxygen species-dependent mitochondrial signaling pathway and suppressing PI3K/Akt signaling pathway in HepG2 cells.
Food Chem Toxicol.2015 Jan;75:173-86.
80.Lai W, Zheng Z, Zhang X, Wei Y, Chu K, Brown J, Hong G, Chen L.
Salidroside-Mediated Neuroprotection is Associated with Induction of Early Growth Response Genes (Egrs) Across a Wide Therapeutic Window.
Neurotox Res.2015 Aug;28(2):108-21.
81.Chu B, Liu F, Li L, Ding C, Chen K, Sun Q, Shen Z, Tan Y, Tan C, Jiang Y.
A benzimidazole derivative exhibiting antitumor activity blocks EGFR and HER2 activity and upregulates DR5 in breast cancer cells.
Cell Death Dis. 2015 Mar 12;6:e1686.
82.Yang J, Yao S.
JNK-Bcl-2/Bcl-xL-Bax/Bak Pathway Mediates the Crosstalk between Matrine-Induced Autophagy and Apoptosis via Interplay with Beclin 1.
Int J Mol Sci. 2015 Oct 27;16(10):25744-58.
83.ang P, Fu S, Cao Z, Liao H, Huo Z, Pan Y, Zhang G, Gao A, Zhou Q.
Oroxin B selectively induces tumor-suppressive ER stress and concurrently inhibits tumor-adaptive ER stress in B-lymphoma cells for effective anti-lymphoma therapy.
Toxicol Appl Pharmacol. 2015 Oct 15;288(2):269-79.
84.Yan J, Liu XH, Han MZ, Wang YM, Sun XL, Yu N, Li T, Su B, Chen ZY.
Blockage of GSK3β-mediated Drp1 phosphorylation provides neuroprotection in neuronal and mouse models of Alzheimer's disease.
Neurobiol Aging. 2015 Jan;36(1):211-27.
85.Li M, Song M, Ren LM, Xiu CY, Liu JY, Zhu YZ, Li YF.
AlCl3 induces lymphocyte apoptosis in rats through the mitochondria-caspase dependent pathway.
Environ Toxicol. 2016 Apr;31(4):385-94.
86.Wang J, Cai J, Huang Y, Ke Q, Wu B, Wang S, Han X, Wang T, Wang Y, Li W, Lao C, Song W, Xiang AP.
Nestin regulates proliferation and invasion of gastrointestinal stromal tumor cells by altering mitochondrial dynamics.
Oncogene. 2016 Jun;34:3139-50.
87.Zhao FL, Qiao PF, Yan N, Gao D, Liu MJ, Yan Y.
Hydrogen Sulfide Selectively Inhibits γ-Secretase Activity and Decreases Mitochondrial Aβ Production in Neurons from APP/PS1 Transgenic Mice.
Neurochem Res. 2016 May;41(5):1145-59.
88.Song E, Tang S, Xu J, Yin B, Bao E, Hartung J.
Lenti-siRNA Hsp60 promote bax in mitochondria and induces apoptosis during heat stress.
Biochem Biophys Res Commun. 2016 Dec 2;481(1-2):125-131.
89.Dong WQ, Chao M, Lu QH, Chai WL, Zhang W, Chen XY, Liang ES, Wang LB, Tian HL, Chen YG, Zhang MX.
Prohibitin overexpression improves myocardial function in diabetic cardiomyopathy.
Oncotarget. 2016 Jan 5;7(1):66-80.
90.Li Q, Tang Y, Qin J, Yi L, Yang Y, Wang J, He J, Su Q, Tan H.
Subcellular localization of DJ-1 in human HL-60 leukemia cells in response to diallyl disulfide treatment.
Mol Med Rep. 2016 Nov;14(5):4666-4672.
91.Kong F, Wang H, Guo J, Peng M, Ji H, Yang H, Liu B, Wang J, Zhang X, Li S.
Hsp70 suppresses apoptosis of BRL cells by regulating the expression of Bcl-, cytochrome C, andcaspase 8/3.
In Vitro Cell Dev Biol Anim. 2016 May;52(5):568-75.
92.Tan G, Liu D, Pan F, Zhao J, Li T, Ma Y, Shen B, Lyu J.
His-87 ligand in mitoNEET is crucial for the transfer of iron sulfur clusters from mitochondria to cytosolicaconitase.
Biochem Biophys Res Commun. 2016 Jan 29;470(1):226-32.
93.Jin F, Qiao C, Luan N, Li H.
Lentivirus-mediated PHLDA2 overexpression inhibits trophoblast proliferation, migration and invasion, and induces apoptosis.
Int J Mol Med. 2016 Apr;37(4):949-57.
94.Feng B, Qiu L, Ye C, Chen L, Fu Y, Sun W.
Exposure to a 50-Hz magnetic field induced mitochondrial permeability transition through the ROS/GSK-3β signaling pathway.
Int J Radiat Biol. 2016;92(3):148-55.
95.Li Z, Liu X, Wang L, Wang Y, Du C, Xu S, Zhang Y, Wang C, Yang C.
The role of PGC-1α and MRP1 in lead-induced mitochondrial toxicity in testicular Sertoli cells.
Toxicology. 2016 Apr 29;355-356:39-48.
96.Li W, Wang Q, Du M, Ma X, Wu L, Guo F, Zhao S, Huang F, Wang H, Qin G.
Effects of overexpressing FoxO1 on apoptosis in glomeruli of diabetic mice and in podocytes cultured inhigh glucose medium.
Biochem Biophys Res Commun. 2016 Sep 16;478(2):612-7.
97.Wang XD, Li CY, Jiang MM, Li D, Wen P, Song X, Chen JD, Guo LX, Hu XP, Li GQ, Zhang J, Wang CH, He ZD.
Induction of apoptosis in human leukemia cells through an intrinsic pathway by cathachunine, a uniquealkaloid isolated from Catharanthus roseus.
Phytomedicine. 2016 Jun 1;23(6):641-53.
98.Wei C, Li H, Wang Y, Peng X, Shao H, Li H, Bai S, Xu C.
Exogenous spermine inhibits hypoxia/ischemia-induced myocardial apoptosis via regulation ofmitochondrial permeability transition pore and associated pathways.
Exp Biol Med (Maywood). 2016 Aug;241(14):1505-15.
99.Wu L, Bao K, Song R, Wang D, Zhang L, Wang W, Zhang W, Bin W.
Development of Novel Erythromycin Derivatives with Inhibitory Activity against Proliferation of TumorCells.
PLoS One. 2016 Jul 22;11(7):e0159503.
100.Yu H, Guan Q, Guo L, Zhang H, Pang X, Cheng Y, Zhang X, Sun Y.
Gypenosides alleviate myocardial ischemia-reperfusion injury via attenuation of oxidative stress andpreservation of mitochondrial function in rat heart.
Cell Stress Chaperones. 2016 May;21(3):429-37.
101.Teng H, Huang Q, Chen L.
Inhibition of cell proliferation and triggering of apoptosis by agrimonolide through MAP kinase (ERK andp38) pathways in human gastric cancer AGS cells.
Food Funct. 2016 Nov 9;7(11):4605-4613.
102.Yang Y, Wang W, Xiong Z, Kong J, Qiu Y, Shen F, Huang Z.
Activation of SIRT3 attenuates triptolide-induced toxicity through closing mitochondrial permeabilitytransition pore in cardiomyocytes.
Toxicol In Vitro. 2016 Aug;34:128-37.
103.Zhang GM, Deng MT, Zhang YL, Fan YX, Wan YJ, Nie HT, Wang ZY, Wang F, Lei ZH.
Effect of PGC-1α overexpression or silencing on mitochondrial apoptosis of goat luteinized granulosa cells.
J Bioenerg Biomembr. 2016 Oct;48(5):493-507.
104.Zhao FL, Qiao PF, Yan N, Gao D, Liu MJ, Yan Y.
Hydrogen Sulfide Selectively Inhibits γ-Secretase Activity and Decreases Mitochondrial Aβ Production inNeurons from APP/PS1 Transgenic Mice.
Neurochem Res. 2016 May;41(5):1145-59.
105.Huang JY, Yuan YH, Yan JQ, Wang YN, Chu SF, Zhu CG, Guo QL, Shi JG, Chen NH.
20C, a bibenzyl compound isolated from Gastrodia elata, protects PC12 cells against rotenone-inducedapoptosis via activation of the Nrf2/ARE/HO-1 signaling pathway.
Acta Pharmacol Sin. 2016 Jun;37(6):731-40.
106.Wo L, Lu D, Gu X.
Knockdown of miR-182 promotes apoptosis via regulating RIP1 deubiquitination in TNF-α-treated triple-negative breast cancer cells.
Tumour Biol. 2016 Oct;37(10):13733-13742.
107.Gong L, Di C, Xia X, Wang J, Chen G, Shi J, Chen P, Xu H, Zhang W.
AKT/mTOR signaling pathway is involved in salvianolic acid B-induced autophagy and apoptosis inhepatocellular carcinoma cells.
Int J Oncol. 2016 Dec;49(6):2538-2548.
108.Gao X, Xu H, Shang J, Yuan L, Zhang Y, Wang L, Zhang W, Luan X, Hu G, Chu H, Zhu T, Jia G.
Ozonized carbon black induces mitochondrial dysfunction and DNA damage.
Environ Toxicol. 2017 Mar;32(3):944-955.
109.Zhang Y, Wu JY, Weng LH, Li XX, Yu LJ, Xu Y.
Valproic acid protects against MPP+-mediated neurotoxicity in SH-SY5Y Cells through autophagy.
Neurosci Lett. 2017 Jan 18;638:60-68.
110.Yang M, Wang B, Gao J, Zhang Y, Xu W, Tao L.
Spinosad induces programmed cell death involves mitochondrial dysfunction and cytochrome C releasein Spodoptera frugiperda Sf9 cells.
Chemosphere. 2017 Feb;169:155-161.
111.Li L, Sun W, Li L, Liu Y, Wu L, Wang F, Zhou Z, Zhang Z, Huang Y.
A pH-responsive sequential-disassembly nanohybrid for mitochondrial targeting.
Nanoscale. 2017 Jan 7;9(1):314-325.
112.Sun W, Li L, Yang QQ, Zhang ZR, Huang Y.
Two birds, one stone: dual targeting of the cancer cell surface and subcellular mitochondria by thegalectin-3-binding peptide G3-C12.
Acta Pharmacol Sin. 2017 Jan 9. doi: 10.1038/aps.2016.137.
113.Wei Y, Yu S, Zhang Y, Zhang Y, Zhao H, Xiao Z, Yao L, Chen S, Zhang J.
NDRG2 promotes adriamycin sensitivity through a Bad/p53 complex atthe mitochondria in breast cancer.
Oncotarget. 2017 Apr 25;8(17):29038-29047.
114.Wang M, Kanako N, Zhang Y, Xiao X, Gao Q, Tetsuya K.
A unique polysaccharide purified from Hericium erinaceus mycelium prevents oxidative stressinduced by H2O2 in human gastric mucosa epithelium cell.
PLoS One. 2017 Jul 24;12(7):e0181546.
115.He Z, Pu L, Yuan C, Jia M, Wang J.
Nutrition deficiency promotes apoptosis of cartilage endplate stem cells ina caspase-independent manner partially through upregulating BNIP3.
Acta Biochim Biophys Sin (Shanghai). 2017 Jan;49(1):25-32.
116.Ying J, Xu J, Shen L, Mao Z, Liang J, Lin S, Yu X, Pan R, Yan C, Li S, Bao Q, Li P.
The Effect of Sodium Fluoride on Cell Apoptosis andthe Mechanism of Human Lung BEAS-2BCells In Vitro.
Biol Trace Elem Res. 2017 Sep;179(1):59-69.
117.Li L, Sun W, Li L, Liu Y, Wu L, Wang F, Zhou Z, Zhang Z, Huang Y.
A pH-responsive sequential-disassembly nanohybrid for mitochondrial targeting.
Nanoscale. 2017 Jan 7;9(1):314-325.
118.Sun W, Li L, Li LJ, Yang QQ, Zhang ZR, Huang Y.
Two birds, one stone: dual targeting ofthe cancer cell surface and subcellular mitochondria by the galectin-3-binding peptide G3-C12.
Acta Pharmacol Sin. 2017 Jun;38(6):806-822.
119.Lin H, Zhao L, Ma X, Wang BC, Deng XY, Cui M, Chen SF, Shao ZW.
Drp1 mediates compression-induced programmed necrosis of rat nucleus pulposus cells by promoting mitochondrial translocation of p53 and nuclear translocation of AIF.
Biochem Biophys Res Commun. 2017 May 20;487(1):181-188.
120.Chang G, Liu J, Qin S, Jiang Y, Zhang P, Yu H, Lu K, Zhang N, Cao L, Wang Y, Li Y, Zhang D.
Cardioprotection by exenatide:A novel mechanism via improving mitochondrial functioninvolving the GLP-1 receptor/cAMP/PKA pathway.
Int J Mol Med. 2017 Dec 12.
121.Wu H, Liu S, Gong J, Liu J, Zhang Q, Leng X, Zhang N, Li Y.
VCPA,a novel synthetic derivative ofα-tocopheryl succinate, sensitizes human gastric cancer to doxorubicin-induced apoptosis via ROS-dependent mitochondrial dysfunction.
Cancer Lett. 2017 May 1;393:22-32.
122.Wu B, Luo H, Zhou X, Cheng CY, Lin L, Liu BL, Liu K, Li P, Yang H.
Succinate-induced neuronal mitochondrial fission and hexokinase II malfunction in ischemicstroke: Therapeutical effects of kaempferol.
Biochim Biophys Acta. 2017 Sep;1863(9):2307-2318.
123.Hua XW, Bao YW, Chen Z, Wu FG.
Carbon quantum dots with intrinsic mitochondrial targeting ability for mitochondria-basedtheranostics.
Nanoscale. 2017 Aug 3;9(30):10948-10960.
124.Gong L, Tang Y1,2 An R, Lin M, Chen L, Du J.
RTN1-C mediates cerebral ischemia/reperfusion injury via ER stress and mitochondria-associated apoptosis pathways.
Cell Death Dis. 2017 Oct 5;8(10):e3080.
125.Huo X, Wang C, Yu Z, Peng Y, Wang S, Feng S, Zhang S, Tian X, Sun C, Liu K, Deng S, Ma X.
Human transporters, PEPT1/2, facilitate melatonin transportation into mitochondria of cancercells: An implication of the therapeutic potential.
J Pineal Res. 2017 May;62(4).
126.Yang Y, Yang H, Yang J, Li L, Xiang B, Wei Q.
The genetically engineered drug rhCNB induces apoptosis via a mitochondrial route in tumorcells.
Oncotarget. 2017 Jul 22;8(39):65876-65888.
127.Wang Y, Guo SH, Shang XJ, Yu LS, Zhu JW, Zhao A, Zhou YF, An GH, Zhang Q, Ma B.
Triptolide induces Sertoli cell apoptosis in mice via ROS/JNK-dependent activation ofthe mitochondrial pathway and inhibition of Nrf2-mediated antioxidant response.
Acta Pharmacol Sin. 2017 Sep 14.
128.Li J, Huang Q, Long X, Guo X, Sun X, Jin X, Li Z, Ren T, Yuan P, Huang X, Zhang H, Xing J.
Mitochondrial elongation-mediated glucose metabolism reprogramming is essential for tumourcell survival during energy stress.
Oncogene. 2017 Aug 24;36(34):4901-4912.
129.Chen J, Chen B, Zou Z, Li W, Zhang Y, Xie J, Liu C.
Costunolide enhances doxorubicin-induced apoptosis in prostate cancer cells via activatedmitogen-activated protein kinases and generation of reactive oxygen species.
Oncotarget. 2017 Nov 21;8(64):107701-107715.
130.Lin H, Ma X, Wang BC, Zhao L, Liu JX, Pu FF, Hu YQ, Hu HZ, Shao ZW.
Edaravone ameliorates compression-induced damage in rat nucleus pulposus cells.
Life Sci. 2017 Nov 15;189:76-83.
131.Wu F, Cao W, Xu H, Zhu M, Wang J, Ke X.
Treatment witha selenium-platinum compound induced T-cell acute lymphoblasticleukemia/lymphoma cells apoptosis throughthe mitochondrial signaling pathway.
Oncol Lett. 2017 Mar;13(3):1702-1710.
132.Yang YL, Li J, Liu K, Zhang L, Liu Q, Liu B, Qi LW.
Ginsenoside Rg5 increases cardiomyocyte resistance to ischemic injury through regulation of mitochondrial hexokinase-II and dynamin-related protein 1.
Cell Death Dis. 2017 Feb 23;8(2):e2625.
133.Chen W, Liu Y, Zhang L, Gu X, Liu G, Shahid M, Gao J, Ali T, Han B.
Nocardia cyriacigeogica from Bovine Mastitis Induced In vitro Apoptosis of Bovine MammaryEpithelial Cells via Activation of Mitochondrial-Caspase Pathway.
Front Cell Infect Microbiol. 2017 May 18;7:194.
134.Liu Z, Gao L, Wang P, Xie Z, Cen S, Li Y, Wu X, Wang L, Su H, Deng W, Wang S, Li D, Li J, Ouyang Y, Wu Y, Shen H.
TNF-α Induced the Enhanced Apoptosis of Mesenchymal Stem Cells in Ankylosing Spondylitis by Overexpressing TRAIL-R2.
Stem Cells Int. 2017;2017:4521324.
135.Song C, Luo B, Gong L.
Resveratrol reduces the apoptosis induced by cigarette smoke extract by upregulating MFN2.
PLoS One. 2017 Apr 13;12(4):e0175009.
136.Xu F, Ren L, Song M, Shao B, Han Y, Cao Z, Li Y.
Fas-and Mitochondria-Mediated Signaling Pathway Involved in Osteoblast Apoptosis Induced by AlCl3.
Biol Trace Elem Res. 2017 Oct 12.
137.Li MD, Cheng WP, Shi MX, Ge TD, Zheng XL, Wu DY, Hu XY, Luo JC, Li FL, Li H.
Role of tRNA selenocysteine 1 associated protein 1 in the proliferation and apoptosis of cardiomyocyte like H9c2 cells.
Mol Med Rep. 2017 Feb;15(2):988-994.
138.Zhang Y, Wu JY, Weng LH, Li XX, Yu LJ, Xu Y.
Valproic acid protects against MPP+-mediated neurotoxicity in SH-SY5Y Cells through autophagy.
Neurosci Lett. 2017 Jan 18;638:60-68.
139.Wang ZB, Zhang S, Li Y, Wang RM, Tong LC, Wang Y, Liu WY, Su DF, Tu Y, Zhang LC, Li L.
LY333531,aPKCβ inhibitor, attenuates glomerular endothelial cell apoptosis inthe early stage of mouse diabetic nephropathy via down-regulating swiprosin-1.
Acta Pharmacol Sin. 2017 Jul;38(7):1009-1023.
140.Qin Y, Meng L, Fu Y, Quan Z, Ma M, Weng M, Zhang Z, Gao C, Shi X, Han K.
SNORA74B gene silencing inhibits gallbladder cancer cells by inducing PHLPP and suppressingAkt/mTOR signaling.
Oncotarget. 2017 Mar 21;8(12):19980-19996.
141.Li XM, Zhang XJ, Dong MX.
Isorhynchophylline Attenuates MPP+-Induced Apoptosis Through Endoplasmic Reticulum Stress- and Mitochondria-Dependent Pathways in PC12 Cells: Involvement of Antioxidant Activity.
Neuromolecular Med. 2017 Dec;19(4):480-492.
142.Huang Z, Yang G, Shen T, Wang X, Li H, Ren D.
Dehydrobruceine B enhances the cisplatin-induced cytotoxicity through regulation ofthe mitochondrial apoptotic pathway in lung cancer A549 cells.
Biomed Pharmacother. 2017 May;89:623-631.
143.Li Y, Zhang L, Qu T, Tang X, Li L, Zhang G.
Conservation and divergence of mitochondrial apoptosis pathway inthe Pacific oyster, Crassostrea gigas.
Cell Death Dis. 2017 Jul 6;8(7):e2915.
144.Qi G, Liu Z, Fan R, Yin Z, Mi Y, Ren B, Liu X.
Athyrium multidentatum (Doll.) Ching extract induce apoptosis via mitochondrial dysfunction and oxidative stress in HepG2 cells.
Sci Rep. 2017 May 23;7(1):2275.
145.Ren T, Zhang H, Wang J, Zhu J, Jin M, Wu Y, Guo X, Ji L, Huang Q, Zhang H, Yang H, Xing J.
MCU-dependent mitochondrial Ca2+ inhibits NAD+/SIRT3/SOD2 pathway to promote ROSproduction and metastasis of HCC cells.
Oncogene. 2017 Oct 19;36(42):5897-5909.
146.Sun J, Jiang L, Lin Y, Gerhard EM, Jiang X, Li L, Yang J, Gu Z.
Enhanced anticancer efficacy of paclitaxel through multistage tumor-targeting liposomesmodified with RGD and KLA peptides.
Int J Nanomedicine. 2017 Feb 27;12:1517-1537.
147.Qie X, Wen D, Guo H, Xu G, Liu S, Shen Q, Liu Y, Zhang W, Cong B, Ma C.
Endoplasmic Reticulum Stress Mediates Methamphetamine-Induced Blood-Brain BarrierDamage.
Front Pharmacol. 2017 Sep 14;8:639.
148.Yang M, Wang B, Gao J, Zhang Y, Xu W, Tao L.
Spinosad induces programmed cell death involves mitochondrial dysfunction and cytochrome Crelease in Spodoptera frugiperda Sf9 cells.
Chemosphere. 2017 Feb;169:155-161.
149.Yang DL, Xu JW, Zhu JG, Zhang YL, Xu JB, Sun Q, Cao XN, Zuo WL, Xu RS, Huang JH, Jiang FN, Zhuo YJ, Xiao BQ, Liu YZ, Yuan DB6, Sun ZL, He HC, Lun ZR, Zhong WD, Zhou WL.
Role of GPR30 in estrogen-induced prostate epithelial apoptosis and benign prostatichyperplasia.
Biochem Biophys Res Commun. 2017 Jun 3;487(3):517-524.
150.Hu X, Kuang Y, Li L, Tang H, Shi Q, Shu X, Zhang Y, Chan FK, Tao Q, He C.
Epigenomic and Functional Characterization of Junctophilin 3 (JPH3) as a Novel Tumor Suppressor Being Frequently Inactivated by Promoter CpG Methylation in Digestive Cancers.
Theranostics. 2017 May 30;7(7):2150-2163.
151.Qin Y, Su Z, Wu Y, Wu C, Jin S, Xie W, Jiang W, Zhou R, Cui J.
NLRP11 disrupts MAVS signalosome to inhibit type I interferon signaling and virus-inducedapoptosis.
EMBO Rep. 2017 Dec;18(12):2160-2171.
152.Jin X, Zheng X, Li F, Liu B, Li H, Hirayama R, Li P, Liu X, Shen G, Li Q.
Fragmentation level determines mitochondrial damage response and subsequently the fate of cancer cells exposed to carbon ions.
Radiother Oncol. 2017 Dec 2. pii: S0167-8140(17)32736-6.
153.Mao XM, Fu QR, Li HL, Zheng YH, Chen SM, Hu XY, Chen QX, Chen QH.
Crocodile choline from Crocodylus siamensis induces apoptosis of human gastric cancer.
Tumour Biol. 2017 Mar;39(3):1010428317694320.
154.Yang J, Zhao JX, Cao Q, Hao L, Zhou D, Gan Z, Ji LN, Mao ZW.
Simultaneously Inducing and Tracking Cancer Cell Metabolism Repression by Mitochondria-Immobilized Rhenium(I) Complex.
ACS Appl Mater Interfaces. 2017 Apr 26;9(16):13900-13912.
155.Fu K, Wang Y, Guo D, Wang G, Ren H.
Familial Parkinson's Disease-Associated L166P Mutant DJ-1 is Cleaved by Mitochondrial SerineProtease Omi/HtrA2.
Neurosci Bull. 2017 Dec;33(6):685-694.
156.Cui G, Li Y, Ding K, Hao S, Wang J, Zhang Z.
Attribution of Bax and mitochondrial permeability transition pore on cantharidin-inducedapoptosis of Sf9 cells.
Pestic Biochem Physiol. 2017 Oct;142:91-101.
157.Wang F, Sun W, Li L, Li L, Liu Y, Zhang ZR, Huang Y.
Charge-Reversible Multifunctional HPMA Copolymers for Mitochondrial Targeting.
ACS Appl Mater Interfaces. 2017 Aug 23;9(33):27563-27574.
158.Tao R, Zhao Y, Chu H, Wang A, Zhu J, Chen X, Zou Y, Shi M, Liu R, Su N, Du J, Zhou HM, Zhu L, Qian X, Liu H, Loscalzo J, Yang Y.
Genetically encoded fluorescent sensors reveal dynamic regulation of NADPH metabolism.
Nat Methods. 2017 Jul;14(7):720-728.
159.Wang Z, Zhou F, Dou Y, Tian X, Liu C, Li H, Shen H, Chen G.
Melatonin Alleviates Intracerebral Hemorrhage-Induced Secondary Brain Injury in Rats viaSuppressing Apoptosis, Inflammation, Oxidative Stress, DNA Damage, and Mitochondria Injury.
Transl Stroke Res. 2018 Feb;9(1):74-91.
160.Wang J, Huang Y, Cai J, Ke Q, Xiao J, Huang W, Li H, Qiu Y, Wang Y, Zhang B, Wu H, Zhang Y, Sui X, Bardeesi ASA, Xiang AP.
A Nestin-Cdk5-Drp1 Axis Regulates Neural Stem/Progenitor Cell Stemness via a Metabolic Shift.
Stem Cells. 2018 Apr;36(4):589-601.
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产品编号
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产品名称
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产品包装
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C3601
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细胞线粒体分离试剂盒
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50-100次
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C3606
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组织线粒体分离试剂盒
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50-100次
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C3609
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线粒体储存液
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50ml
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C3702
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红细胞裂解液
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120ml
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P0027
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细胞核蛋白与细胞浆蛋白抽提试剂盒
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50次
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P0028
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细胞核蛋白与细胞浆蛋白抽提试剂盒
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100次
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P0033
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细胞膜蛋白与细胞浆蛋白提取试剂盒
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100次
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产品编号
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产品名称
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产品包装
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C3702
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红细胞裂解液
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120ml
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P0013
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Western及IP细胞裂解液
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100ml
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P0013B
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RIPA裂解液(强)
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100ml
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P0013C
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RIPA裂解液(中)
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100ml
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P0013D
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RIPA裂解液(弱)
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100ml
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P0013E
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RIPA裂解液(强中弱套装)
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共150ml
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P0013F
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NP-40裂解液
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100ml
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P0013G
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SDS裂解液
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100ml
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P0013J
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Western及IP细胞裂解液(无抑制剂)
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100ml
|
|
P0013K
|
RIPA裂解液(强, 无抑制剂)
|
100ml
|
|
P0027
|
细胞核蛋白与细胞浆蛋白抽提试剂盒
|
50次
|
|
P0028
|
细胞核蛋白与细胞浆蛋白抽提试剂盒
|
100次
|
|
P0033
|
细胞膜蛋白与细胞浆蛋白提取试剂盒
|
100次
|
|
ST206
|
Lysozyme/溶菌酶(>20KU/mg)
|
0.5g
|
|
P0603
|
SABC-HRP Kit (IHC,
ICC, Blotting & ELISA)
|
1000次
|
|
P0606
|
SABC-AP Kit (IHC,
ICC, Blotting&ELISA)
|
1000次
|
|
P0612
|
SABC-HRP Kit with
Anti-Mouse IgG (IHC&ICC)
|
500次
|
|
P0615
|
SABC-HRP Kit with
Anti-Rabbit IgG (IHC&ICC)
|
500次
|
|
P0625
|
SABC-AP Kit with
Anti-Mouse IgG (IHC&ICC)
|
500次
|
|
P0628
|
SABC-AP Kit with
Anti-Rabbit IgG (IHC&ICC)
|
500次
|
|
R0121-25ml
|
AllProtect™动物组织核酸、蛋白稳定保存液
|
25ml
|
|
R0121-100ml
|
AllProtect™动物组织核酸、蛋白稳定保存液
|
100ml
|
|
C3601
|
细胞线粒体分离试剂盒
|
50-100次
|
|
C3606
|
组织线粒体分离试剂盒
|
50-100次
|
|
S3090
|
细胞与组织裂解液(一氧化氮检测用)
|
100ml
|
|
P1005
|
蛋白酶抑制剂混合物(通用型, 100X)
|
1ml
|
|
ST506
|
PMSF (100mM)
|
10ml
|
|
P1006
|
蛋白酶抑制剂混合物(通用型, 100X)
|
5ml
|
|
P1008
|
蛋白酶抑制剂混合物(通用型, 质谱兼容,
50X)
|
1ml
|
|
P1009
|
蛋白酶抑制剂混合物(通用型, 质谱兼容,
50X)
|
5ml
|
|
P1010
|
蛋白酶抑制剂混合物(哺乳动物样品抽提用,
100X)
|
1ml
|
|
P1011
|
蛋白酶抑制剂混合物(哺乳动物样品抽提用,
100X)
|
5ml
|
|
P1015
|
蛋白酶抑制剂混合物(植物样品抽提用,
100X)
|
1ml
|
|
P1016
|
蛋白酶抑制剂混合物(植物样品抽提用,
100X)
|
5ml
|
|
P1020
|
蛋白酶抑制剂混合物(真菌或酵母抽提用,
100X)
|
1ml
|
|
P1021
|
蛋白酶抑制剂混合物(真菌或酵母抽提用,
100X)
|
5ml
|
|
P1025
|
蛋白酶抑制剂混合物(细菌抽提用,
100X)
|
1ml
|
|
P1026
|
蛋白酶抑制剂混合物(细菌抽提用,
100X)
|
5ml
|
|
P1030
|
蛋白酶抑制剂混合物(His-Tag蛋白纯化用,
100X)
|
1ml
|
|
P1031
|
蛋白酶抑制剂混合物(His-Tag蛋白纯化用,
100X)
|
5ml
|
|
P1045
|
蛋白酶磷酸酶抑制剂混合物(通用型 ,
50X)
|
各2ml
|
|
P1046
|
蛋白酶磷酸酶抑制剂混合物(通用型,
50X)
|
各10ml
|
|
P1048
|
蛋白酶磷酸酶抑制剂混合物(通用型,
质谱兼容, 50X)
|
各1ml
|
|
P1049
|
蛋白酶磷酸酶抑制剂混合物(通用型,
质谱兼容, 50X)
|
各5ml
|
|
P1050
|
蛋白酶磷酸酶抑制剂混合物(哺乳动物样品抽提用,
50X)
|
各2ml
|
|
P1051
|
蛋白酶磷酸酶抑制剂混合物(哺乳动物样品抽提用,
50X)
|
各10ml
|
|
P1055
|
蛋白酶磷酸酶抑制剂混合物(植物样品抽提用,
50X)
|
各2ml
|
|
P1056
|
蛋白酶磷酸酶抑制剂混合物(植物样品抽提用,
50X)
|
各10ml
|
|
P1060
|
蛋白酶磷酸酶抑制剂混合物(真菌或酵母抽提用,
50X)
|
各2ml
|
|
P1061
|
蛋白酶磷酸酶抑制剂混合物(真菌或酵母抽提用,
50X)
|
各10ml
|
|
P1065
|
蛋白酶磷酸酶抑制剂混合物(细菌抽提用,
50X)
|
各2ml
|
|
P1066
|
蛋白酶磷酸酶抑制剂混合物(细菌抽提用,
50X)
|
各10ml
|
|
P1081
|
磷酸酶抑制剂混合物A (50X)
|
2ml
|
|
P1082
|
磷酸酶抑制剂混合物A (50X)
|
10ml
|
|
P1086
|
磷酸酶抑制剂混合物B (50X)
|
2ml
|
|
P1087
|
磷酸酶抑制剂混合物B (50X)
|
10ml
|
|
P1091
|
磷酸酶抑制剂混合物C (50X)
|
2ml
|
|
P1092
|
磷酸酶抑制剂混合物C (50X)
|
10ml
|
|
P1096
|
磷酸酶抑制剂混合物D (50X)
|
2ml
|
|
P1097
|
磷酸酶抑制剂混合物D (50X)
|
10ml
|
|
P1112
|
去乙酰化酶抑制剂混合物(100X)
|
1ml
|
|
P1113
|
去乙酰化酶抑制剂混合物(100X)
|
5ml
|