


| Feature Nucleotide | Position |
| CMV promoter | 1-602 |
| T3 promoter and T3 primer binding site | 620-639 |
| multiple cloning site | 680-740 |
| c-Flag tag | 741-764 |
| T7 promoter and T7 primer binding site | 817-838 |
| SV40 polyA signal | 850-1233 |
| f1 origin of ss-DNA replication | 1371-1675 |
| bla promoter | 1700-1824 |
| SV40 promoter | 1844-2182 |
| neomycin/kanamycin resistance ORF | 2217-3008 |
| HSV-thymidine kinase (TK) polyA signal | 3009-3467 |
| pUC origin | 3596-4263 |
pCMV-C-Flag的多克隆位点的详细图谱如下:
| XmaIPstI | ||||||||
| SacI Small BamHI HindIII | ||||||||
| 651 | GAGCTCCACC | GCGGTGGCGG | CCGCTCTAGC | CCGGGCGGAT | CCAAGCTTCT | |||
| CTCGAGGTGG | CGCCACCGCC | GGCGAGATCG | GGCCCGCCTA | GGTTCGAAGA | ||||
| Flag | ||||||||
| EcoRI EcoRV SacI BglII XhoI XbaI |
|
|||||||
| 701 | GCAGGAATTC | GATATCGTCG | ACAGATCTCT | CGAGTCTAGA |
|
|||
| CGTCCTTAAG | CTATAGCAGC | TGTCTAGAGA | GCTCAGATCT |
|
||||
|
||||||||
| D D D K ApaI | ||||||||
| 751 | ATGACGACGA |
|
CCCGGTACCT | TAATTAATTA | AGGTACCAGG | |||
| TACTGCTGCT |
|
GGGCCATGGA | ATTAATTAAT | TCCATGGTCC | ||||
pCMV-C-Flag中没有的酶切位点(Restriction enzymes that do not cut pCMV-C-Flag)包括:
| Afl II | Age I | Ahd I | Asc I | Bbs I | Bbv II | Blp I |
| Bsg I | BsiW I | BsmB I | BspM II | BsrG I | BssH II | Bst1107 I |
| BstE II | Ear I | Eco47 III | Eco72 I | EcoN I | Esp I | Fse I |
| Nru I | PflM I | Pme I | Pml I | PpuM I | Psp1406 I | Sap I |
| Sca I | Spe I | |||||
pCMV-C-Flag中的单酶切位点(Restriction enzymes that cut pCMV-C-Flag once)包括:
| Nde I | CA`TA,TG | 241 | Pvu I | CG,AT`CG | 851 | |
| SnaB I | TAC|GTA | 347 | Bcl I | T`GATC,A | 1005 | |
| Nhe I | G`CTAG,C | 598 | Mun I | C`AATT,G | 1098 | |
| Sac I | G,AGCT`C | 656 | Hpa I | GTT|AAC | 1111 | |
| Sac II | CC,GC`GG | 663 | Mlu I | A`CGCG,T | 1234 | |
| BstX I | CCAN,NNNN`NTGG | 664 | Dra III | CAC,NNN`GTG | 1464 | |
| Not I | GC`GGCC,GC | 669 | Sfi I | GGCCN,NNN`NGGCC | 2121 | |
| PspA I | C`CCGG,G | 681 | BseR I | GAGGAG 16/14 | 2164 | |
| Xma I | C`CCGG,G | 681 | Stu I | AGG|CCT | 2167 | |
| Srf I | GCCC|GGGC | 683 | Cla I | AT`CG,AT | 2186 | |
| Sma I | CCC|GGG | 683 | Kas I | G`GCGC,C | 2345 | |
| BamH I | G`GATC,C | 688 | Nar I | GG`CG,CC | 2346 | |
| HinD III | A`AGCT,T | 694 | Ehe I | GGC|GCC | 2347 | |
| Pst I | C,TGCA`G | 704 | Bbe I | G,GCGC`C | 2349 | |
| EcoR I | G`AATT,C | 706 | Msc I | TGG|CCA | 2428 | |
| EcoR V | GAT|ATC | 714 | Tth111 I | GACN`N,NGTC | 2464 | |
| Sal I | G`TCGA,C | 718 | BsrD I | GCAATG, 8 | 2579 | |
| Acc I | GT`MK,AC | 719 | Bsp1286 I | G,DGCH`C | 2649 | |
| Bgl II | A`GATC,T | 724 | Rsr II | CG`GWC,CG | 2862 | |
| PaeR7 I | C`TCGA,G | 730 | BsiC I | TT`CG,AA | 3028 | |
| Xho I | C`TCGA,G | 730 | BstB I | TT`CG,AA | 3028 | |
| Xba I | T`CTAG,A | 736 | Bsa I | GGTCTC 7/11 | 3335 | |
| Bsp120 I | G`GGCC,C | 769 | HgiE II | ACCNNNNNNGGT-1/13 | 3675 | |
| Apa I | G,GGCC`C | 773 | ApaL I | G`TGCA,C | 3950 |
pCMV-C-Flag质粒中对于插入片段进行测序时,推荐使用的正向测序引物T3和反向测序引物T7的序列如下:
T3 primer(620-639): 5′AATTAACCCTCACTAAAGGG 3′
T7 primer(817-838): 5′GTAATACGACTCACTATAGGGC 3′
pCMV-C-Flag的全序列信息: D2632 pCMV-C-Flag-seq.txt
包装清单:
| 产品编号 | 产品名称 | 包装 |
| D2632-1μg | pCMV-C-Flag | 1μg |
| D2632-100μg | pCMV-C-Flag | 100μg |
| — | 说明书 | 1份 |
保存条件:
-20℃保存。
注意事项:
本质粒未经碧云天书面许可不得用于任何商业用途,也不得移交给订货人所在实验室外的任何个人或单位。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。
|
产品编号
|
产品名称
|
产品包装
|
|
D2602-1μg
|
pCMV-Blank
|
1μg
|
|
D2602-100μg
|
pCMV-Blank
|
100μg
|
|
D2621-1μg
|
pCMV-C-BFP (蓝色荧光蛋白)
|
1μg
|
|
D2621-100μg
|
pCMV-C-BFP (蓝色荧光蛋白)
|
100μg
|
|
D2623-1μg
|
pCMV-C-CFP (青色荧光蛋白)
|
1μg
|
|
D2623-100μg
|
pCMV-C-CFP (青色荧光蛋白)
|
100μg
|
|
D2624-1μg
|
pCMV-C-DsRed (红色荧光蛋白)
|
1μg
|
|
D2624-100μg
|
pCMV-C-DsRed (红色荧光蛋白)
|
100μg
|
|
D2626-1μg
|
pCMV-C-EGFP (绿色荧光蛋白)
|
1μg
|
|
D2626-100μg
|
pCMV-C-EGFP (绿色荧光蛋白)
|
100μg
|
|
D2628-1μg
|
pCMV-C-mCherry
(红色荧光蛋白)
|
1μg
|
|
D2628-100μg
|
pCMV-C-mCherry
(红色荧光蛋白)
|
100μg
|
|
D2630-1μg
|
pCMV-C-YFP (黄色荧光蛋白)
|
1μg
|
|
D2630-100μg
|
pCMV-C-YFP (黄色荧光蛋白)
|
100μg
|
|
D2632-1μg
|
pCMV-C-Flag
|
1μg
|
|
D2632-100μg
|
pCMV-C-Flag
|
100μg
|
|
D2639-1μg
|
pCMV-C-HA
|
1μg
|
|
D2639-100μg
|
pCMV-C-HA
|
100μg
|
|
D2650-1μg
|
pCMV-C-His
|
1μg
|
|
D2650-100μg
|
pCMV-C-His
|
100μg
|
|
D2672-1μg
|
pCMV-C-Myc
|
1μg
|
|
D2672-100μg
|
pCMV-C-Myc
|
100μg
|
|
D2701-1μg
|
pCMV-N-BFP (蓝色荧光蛋白)
|
1μg
|
|
D2701-100μg
|
pCMV-N-BFP (蓝色荧光蛋白)
|
100μg
|
|
D2703-1μg
|
pCMV-N-CFP (青色荧光蛋白)
|
1μg
|
|
D2703-100μg
|
pCMV-N-CFP (青色荧光蛋白)
|
100μg
|
|
D2705-1μg
|
pCMV-N-DsRed (红色荧光蛋白)
|
1μg
|
|
D2705-100μg
|
pCMV-N-DsRed (红色荧光蛋白)
|
100μg
|
|
D2707-1μg
|
pCMV-N-EGFP (绿色荧光蛋白)
|
1μg
|
|
D2707-100μg
|
pCMV-N-EGFP (绿色荧光蛋白)
|
100μg
|
|
D2711-1μg
|
pCMV-N-mCherry
(红色荧光蛋白)
|
1μg
|
|
D2711-100μg
|
pCMV-N-mCherry
(红色荧光蛋白)
|
100μg
|
|
D2716-1μg
|
pCMV-N-YFP (黄色荧光蛋白)
|
1μg
|
|
D2716-100μg
|
pCMV-N-YFP (黄色荧光蛋白)
|
100μg
|
|
D2722-1μg
|
pCMV-N-Flag
|
1μg
|
|
D2722-100μg
|
pCMV-N-Flag
|
100μg
|
|
D2733-1μg
|
pCMV-N-HA
|
1μg
|
|
D2733-100μg
|
pCMV-N-HA
|
100μg
|
|
D2737-1μg
|
pCMV-N-His
|
1μg
|
|
D2737-100μg
|
pCMV-N-His
|
100μg
|
|
D2756-1μg
|
pCMV-N-Myc
|
1μg
|
|
D2756-100μg
|
pCMV-N-Myc
|
100μg
|
|
D2802
|
pFastBac1-EGFP
(杆状病毒包装阳性对照质粒)
|
1μg
|