ELISA Kit

人白介素10(IL-10)ELISA KIT

Human Interleukin 10,IL-10 ELISA KIT

CSB-E04593h

CSIF, IL-10, IL10A, MGC126450, MGC126451, TGIF, cytokine synthesis inhibitory factor

96T

3600

Homo sapiens (Human)

interleukin 10

IL10

12.5 pg/mL-800 pg/mL

3.12 pg/mL

1-5h

50-100ul

450 nm

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

To assess the linearity of the assay, samples were spiked with high concentrations of human IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		92
		Range %		85-100
1:2		Average %		100
		Range %		91-105
1:4		Average %		97
		Range %		92-102
1:8		Average %		93
		Range %		86-98

The recovery of human IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		89-100

quantitative

Immunology