ELISA Kit

小鼠白介素17(IL-17)ELISA Kit

Mouse Interleukin 17,IL-17 ELISA Kit

CSB-E04608m

CTLA8, IL-17, IL-17A, IL17, cytotoxic T-lymphocyte-associated antigen 8|cytotoxic T-lymphocyte-associated protein 8|cytotoxic T-lymphocyte-associated serine esterase 8|interleukin 17 (cytotoxic T-ly

96T

3200

Mus musculus (Mouse)

interleukin 17A

IL17A

47 pg/mL-3000 pg/mL

11.75 pg/mL

1-5h

50-100ul

450 nm

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 3000 2.807 2.909 2.858 2.690 1500 2.386 2.334 2.360 2.192 750 1.568 1.603 1.586 1.418 375 0.835 0.874 0.855 0.687 187.5 0.442 0.459 0.451 0.283 94 0.317 0.334 0.326 0.158 47 0.254 0.243 0.249 0.081 0 0.162 0.174 0.168

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		95
		Range %		89-101
1:2		Average %		95
		Range %		90-100
1:4		Average %		88
		Range %		81-99
1:8		Average %		90
		Range %		81-98

The recovery of mouse IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		93		88-100
EDTA plasma (n=4)		94		90-99

quantitative

Immunology